Since the 1996 Olympics in Atlanta, the Analytical Chemistry and Protein Profiling/2D Gel Laboratory has operated as a shared facility between the Department of Pharmacology and Toxicology and the Clinical Research Center. In addition, investigators from the NASA Space Medicine and Life Sciences Research Center at Morehouse School of Medicine also extensively used the analytical chemistry laboratory. The CRC has recently realigned and consolidated the analytical core with the proteomics core laboratories to become the Analytical and Protein-Profiling Core, a virtual umbrella or super core, similar to other institutional research cores. For both basic and clinical investigators, the Analytical and Protein Profiling Laboratory will be a multifunctional resource providing analytical consultation, help in the preparation of manuscripts and proposals as well as sample analysis. This administrative structure ensures uniformity in investigator utilization, a common platform for marketing of services, thus promoting access, and increased utilization. The revised structure also allows investigators to access and view all MSM research core labs “at a glance”. Problems arising due to variations in methods between laboratories can be avoided.
The Analytical Chemistry and Protein Profiling Core is comprised of three primary labs:
Protein Profiling/2D Gel
2D Gel Electrophoresis Lab
Analytical Chemistry Lab (ACL) provides custom analytical support for clinical, basic and transitional research. The services that the ACL routinely performs include analysis via: 1) HPLC, 2) ELISA and colorimetric testing and 3) Electrolytes analyses. In addition to providing analytical support, the ACL provides consults on analytical techniques, sample requirements, etc. to the investigators throughout the project (from concept to completion).
Protein Profiling Lab offers services such as protein identification and analysis of protein modifications. It houses a Surface Enhanced Laser Desorption-Ionization (SELDI) mass spectrometer, and a Thermo-Finnigan LTQ LC/MS/MS system. SELDI is a mass spectrometry technique used to analyze proteins and other complex mixtures of biomolecules. This core lab also has a BioPlex bead sorter, which allows the analysis of multiplex assays such as ELISAs from a single, small volume sample.
2D Gel Electrophoresis Lab works in conjunction with the Protein Profiling lab and offers differential protein expression analysis by both gel and LC analysis. Within this lab is a BioRad automated spot picker (Proteome Works) and a BioRad gel imager (Molecular imager FX).
For more information and service charges, contact:
Dr. Michael Powell
Proteomics Core Leader
Phone: (404) 752-1582
Dr. Winston Thompson
2D Gel Electrophonesis Core Leader
Phone: (404) 752-1715
Phone: (404) 756-5707